2S Albumin from Crambe (Crambe Abyssinica) against Trypanosoma Cruzi
Araujo, J.S.; Galossi F.S, Carvalho, L.P.; Melo, E.J.T; Machado, O.L.T
Abstract
Introduction: Seed storage proteins provide a store of amino acids for use during germination and seedling growth but can also play a defensive role in their insecticidal and antimicrobial properties. We determined the potential toxic activity of a 2S albumin, isolated, from crambe seeds, on the growth of Trypanosoma cruzi, a protozoan that causes Chagas disease. Human Chagas disease is a tropical illness that affects 6-8 million people worldwide, causing approximately 8000 deaths in 2015.
Methods: Crambe seed proteins were extracted in phosphate buffer and chromatographically partitioned. The fractions were submitted to a growth-inhibition assay against T. cruzi epimastigotes and amastigotes in LLC-MK2 host cells. The cells were examined by light and transmission electron microscopy.
Results: The 2S albumin protein was not toxic to LLC-MK2 cells at 500 μg/mL but eliminated 70% of the epimastigotes after 48 h and reduced the number of amastigotes infected cells. Amastigotes are the protozoa form found in the bloodstream or within host cells. Morphological and ultrastructural analyses demonstrated the parasite's deformation and organelles, including membrane damage and fragmentation of intracellular amastigotes.
Conclusions: 2S albumin from crambe exhibits activity against both amastigote and epimastigoteT. cruzi forms and may be sources of new compounds clinically active against T. cruzi.
Full Text: PDF DOI: 10.15640/ijhs.v11n1a6
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