International Journal of Health Sciences

Effect various type of exercise to Insr gene expression, skeletal muscle insulin receptor and insulin Resistance on Diabetes Mellitus Type-2 model Rats
Yetty Machrina, Harun AL-Rasyid Damanik, Ambrosius Purba, Dharma Lindarto

Abstract
Physical activity is one of type-2 Diabetes mellitus(T2DM)management. Previous studies proved that aerobic training could improve insulin receptor sensitivity and insulin resistance. Aerobic training can be continuous and interval training. This study aimed to analyse the effect of various type of exercise to Insrgene expression, skeletal muscle insulin receptor, and HOMA-IR changes. It was an experimental study, using twenty male Wistar, aged eight weeks, weight 150-180 gram, T2DM model. We divided Rats into five groups, one group as control (sedentary) and four groups as treated groups, i.e., moderate continuous training (MCT), severe continuous training (SCT), slow interval training (SIT) and fast interval training (FIT). All treatment groups ran on the treadmill three times a week for eight weeks. Blood glucose level and HOMA-IR were checked before and after the exercise assignment. Insr gene expression was assessed by qPCR, skeletal muscle insulin receptor determined by IHC, fasting insulin was examined by ELISA, and blood glucose level was checked by using a spectrophotometer.The result found that Insr gene expression decline, Insulin receptor distribution on skeletal muscle increased, insulin resistance and fasting blood glucose decreasedin treated groups after eight weeks of exercises compared with control (p<0,05).In continuous groups, MCT was better than SCT to decline Insrgene expression, while SCT tends to better than MCT to increase skeletal muscle insulin receptor and decreased insulin resistance. In interval groups, FIT was tending to a better model than SIT to decreased Insrgene expression and increased skeletal muscle insulin receptor, while SIT tends to better decreased insulin resistance than FIT. Among the four models, SIT has the lowest insulin resistance at the post-test. The lowest Insrgene expression and highest skeletal muscle insulin receptor distribution were found in the FIT group. In conclusion, there was the effect of type of exercise to Insr gene expression, skeletal muscle insulin receptor, insulin receptor and fasting blood glucose in type-2 DM model rats. Fast interval training was the best model to decline Insrgene expression and increased skeletal muscle insulin receptor. Slow interval training was the best model for decreased insulin resistance.

Full Text: PDF     DOI: 10.15640/ijhs.v6n4a8