A Pilot Study of Increased Gene Expression of Growth Differentiation Factor 15 and Telomerase Reverse Transcriptase in the Middle-Aged with Acute Coronary Artery Disease
Abdelsabour MA, Idriss NK, Blann AD, Mosa AA, Fouad DA, Amal AM, Ashry A, Sayed SA, Nasreldin E, Hassan SA, Elnaggar MG, Meki AM, Hassen HA ,Gaber MA
Abstract
Background: Growth Differentiation Factor 15 (GDF15) and Telomerase Reverse Transcriptase (TERT) may be involved in cardiovascular disease. We hypothesised altered expression of the genes for these molecules in acute coronary artery disease (CAD).
Methods: Venous blood was obtained from 53 patients (27 with diabetes) presenting with an acute coronary syndrome and subsequently shown to have CAD, and from 46 age and sex matched controls free of cardiovascular disease and its risk factors. Relative expression of leukocyte transcriptome GAPDH, GDF15 and TERT were determined by RT-PCR and quantified by quantitation-comparative Ct (ΔCt).
Results: Compared to controls, mean (95% CI) expression of GDF15 in the patients was 1.38 (1.13-1.49) (p<0.001), and of TERT was 1.12 (1.04-1.20) p=0.003), with GDF15 being greater than that of TERT (p<0.001). There was no difference in GDF15 expression in 26 patients free of diabetes (1.6 [1.42-1.78]) versus 27 with diabetes (1.6 [1.29-1.91]) (p=0.996), and no difference in the TERT expression in patients free of diabetes (1.19 [1.06-1.33]) compared to those with diabetes (1.25 [0.98-1.50]) (p=0.739).
Conclusions: GDF15 and TERT expressions are both increased in CAD and in CAD+diabetes, with no difference between the patient groups. These genes may have roles in the pathogenesis of acute CAD.
Full Text: PDF DOI: 10.15640/ijhs.v11n2a10
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