International Journal of Health Sciences

The Underlying Mechanisms for Resveratrol’ Superinduction Effects
Wei Li

Abstract
The superinduction effects of resveratrol was defined by its ability to activate estrogen response element (ERE) regulated luciferase reporter genes 2-3 fold higher than observed with 17β-estradiol (E2). In HeLa cells, the superinduction effects of resveratrol with transfected estrogen receptor α (ERα) are cell density-dependent, but they are cell density-independent with ERβ. The superinduction effects of resveratrol are also shown to be independent of both ER and ERE. The extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) pathway is critical for the superinduction effects of resveratrol in HeLa cells. The ERK1/2 inhibitor U0126 abolishes the superinduction effects of resveratrol, suggesting that this pathway is vital for resveratrol’s superinduction effects. Western blotting results verified that resveratrol could activate ERK1/2 significantly 30 minutes after treatment. Resveratrol shows no effects on histone deacetylase (HDAC) activities both in vivo and in vitro by observing histone H4 acetylation level changes. Our study suggests that the ERK1/2 pathway is involved in the superinduction effects of resveratrol in HeLa cells.

Full Text: PDF     DOI: 10.15640/ijhs.v3n1a3